Surface Plasmon Based Substrate for Enhanced Fluorescence Live Cell Imaging
نویسندگان
چکیده
1 Micro and Nanotechnology Laboratory, 2 Institute for Genomic Biology, 3 Beckman Institute for Advanced Science and Technology, University of Illinois, Urbana –Champaign, IL, 61801 USA ABSTRACT We have created a randomly distributed nanocone substrate on silicon and coated with silver for surface-plasmonenhanced fluorescence detection and cell imaging. We observed three dimensional fluorescence enhancement on our substrate evidenced from the confocal fluorescence imaging of Chinese Hamster Ovary (CHO) cells and transfected HeLa cells grown on the substrate. The fluorescence intensity was amplified more than 100 fold as compared to glass substrate. Strong scattering within nanostructured area coupled with random total internal reflection inside the cell resulted in the observed enhancement in fluorescence further away from the substrate surface.
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